RESUMO
A novel actinobacterium designated TRM 46509T was isolated from a salt water beach at Kalpin, Xinjiang, north-west China. The strain was aerobic and Gram-stain-positive, with an optimum NaCl concentration for growth of 1â% (w/v). The isolate formed sparse aerial mycelium and produced spiral spores at the end of the aerial mycelium on Gauze's No. 1 medium. The isolate contained ll-diaminopimelic acid as the diagnostic diamino acid and ribose as the major whole-cell sugar. The polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannosides and an unidentified phospholipid. The predominant menaquinones were MK-9(H2), MK-9(H6) and MK-9(H8). The major fatty acids were C16:0, iso-C16â:â0, anteiso-C15â:â0, iso-C15â:â0 and iso-C14â:â0. The G+C content of the DNA was 69.3 mol%. Phylogenetic analysis showed that strain TRM 46509T shared 16S rRNA gene sequence similarity of 97.6â% with the closest described species Streptomyces tacrolimicus ATCC 55098T. On the basis of evidence from this polyphasic study, strain TRM 46509T should be designated as representing a novel species of the genus Streptomyces, for which the name Streptomyces kalpinensis sp. nov. is proposed. The type strain is TRM 46509T (=CCTCC AA 2015028T=KCTC 39667T).
Assuntos
Filogenia , Água do Mar/microbiologia , Streptomyces/classificação , Actinobacteria/genética , Técnicas de Tipagem Bacteriana , Composição de Bases , Praias , China , DNA Bacteriano/genética , Ácido Diaminopimélico/química , Ácidos Graxos/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Streptomyces/genética , Streptomyces/isolamento & purificação , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMO
A novel actinomycete strain, designated TRM 46515T, was isolated from a salt water beach at Awat, Xinjiang, Northwest China, and characterized using polyphasic taxonomy. Comparison of 16S rRNA gene sequences showed that strain TRM 46515T is a member of the genus Streptomyces, exhibiting highest similarity with Streptomyces qinglanensis 172205T (98.32 %). However, DNA-DNA relatedness and phenotypic data readily distinguished strain TRM 46515T from phylogenetically related type strains. The G+C content of the DNA was 70.40 mol%. Whole-cell hydrolysates of strain TRM 46515T were found to contain ll-diaminopimelic acid as the diagnostic diamino acid and ribose was the major whole-cell sugar. The major fatty acids identiï¬ed were anteiso-C17 : 0, anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0. The main menaquinone was MK-9(H8) and the polar lipids were identiï¬ed as diphosphatidylglycerol, phosphatidyl ethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and one unknown glycolipid. On the basis of these phenotypic, chemotaxonomic and phylogenetic data, strain TRM 46515T should be designated as a representative of a novel species of the genus Streptomyces, for which the name Streptomyces litoralis sp. nov. is proposed. The type strain is TRM 46515T (=CCTCC AA 2015040T=KCTC 39729T).
